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. 2000 Feb 29;97(6):2491–2496. doi: 10.1073/pnas.050586597

Figure 1.

Figure 1

Inhibition of copper-dependent CUP1 induction by DEA/NO. Late log-phase cultures of 217CUP in SD −ura −Cu plus 0.1 mM BCS were used. (A) Time course of inhibition of CUP1 induction by DEA/NO. The cells were incubated with (closed circle) or without (open circle) 1 mM DEA/NO for indicated time at 30°C with shaking (275 rpm). The cells were then homogenized and assayed for β-galactosidase activity. (B) Dose-dependent inhibition of CUP1 induction by DEA/NO. The cells were exposed to 0–1 mM of DEA/NO (closed square) or decomposed DEA/NO (open square) for 10 min at 30°C with shaking (275 rpm). After washing with DDW twice, the cells were resuspended in SD −ura plus 0.1 mM CuSO4 to start induction of CUP1-lacZ and were incubated at 30°C for 60 min. Representative experiments are shown.