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Time-dependent recovery of CUP1 induction after inhibition by DEA/NO. Late log phase 217CUP cells were incubated with (closed circle) or without (open circle) DEA/NO (1 mM) at 30°C for 10 min with shaking (275 rpm). After washing, the cells were resuspended in SD −ura plus 0.1 mM CuSO4 to induced CUP1-lacZ for 0–8 hours. β-galactosidase activity was then determined. Representative experiments are shown.
