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Kinetics of the reconstitution of apo-SDH with the α-domain (■) and β-domain (□). Clusters were incubated with apo-SDH (1.7 μM) at a molar ratio between zinc in the clusters and apo-SDH of 1.4 in 0.2 M Tris⋅HCl, pH 7.4. Aliquots (10 μl) were periodically withdrawn from the mixtures and assayed for SDH activity.
