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. 2000 Mar 14;97(6):2567–2572. doi: 10.1073/pnas.97.6.2567

Figure 5.

Figure 5

Fractionation of BIG1 and BIG2 on Sepharose CL-6B. Cytosol (16 mg protein) from HeLa cells was fractionated on Sepharose CL-6B. Samples of fractions were subjected to SDS/PAGE, followed by Western blotting using anti-BIG1 and anti-BIG2 IgGs. Immunoreactive bands were quantified by densitometry; amounts of BIG1 and BIG2 are recorded as percentage of the total of each recovered. Elution positions of standard proteins are indicated. Th, thyroglobulin (669 kDa); Fe, ferritin (440 kDa); Ca, catalase (232 kDa); Al, aldolase (158 kDa). Data from a replicate experiment were similar.