Skip to main content
. 2000 Feb 25;97(6):2573–2578. doi: 10.1073/pnas.040337597

Figure 5.

Figure 5

Tumorigenicity of rat AT2.1 and AT3.1 prostate carcinoma cells transfected with sNRP1. AT2.1 cells expressing sNRP1 (A, C, and E) or control cells with neo vector alone (B, D, and F) were injected s.c. in rats. AT3.1 cells expressing sNRP1 (G) or control cells with neo vector alone (H) were injected orthotopically into rat prostates. (A) Hematoxylin/eosin (H&E) staining. Tumors of cells expressing sNRP1 have a rim (shown between arrows) of normal-looking tumor and a center filled with blood and nonviable cells. (B) H&E staining. Control tumors are solid throughout. (C) PCNA (brown). Proliferating cells are found only at the outer edge of AT2.1-sNRP1 tumors. (D) PCNA. AT2.1-neo tumors contain proliferating cells throughout. (E–H) TUNEL assay (brown). The centers of tumors expressing sNRP1 are full of apoptotic cells (E and G) whereas control tumors have very few areas of apoptosis (F and H). (Bars = 5 mm.) Similar results were obtained in three independent AT2.1-sNRP1 and AT3.1-sNRP1 clones, each injected into animals in duplicate.