Figure 5.

(A) mik1-MYC cells [in wild-type (wt), rad3-d, cds1-d, or chk1-d background] were synchronized in G₂ by elutriation, and aliquots were collected every 20 min. For each time point, an aliquot was assayed for septation index, Mik1-Myc levels (50 μg of total protein, pellet fraction), and DNA content (FACS). A second aliquot was treated with 250 Gy of irradiation, allowed to recover (for 45 min at 30°C) and similarly assayed for Mik1-Myc levels and DNA content. (B) cdc25.22 mik1-MYC cells (in wild-type, rad3-d, cds1-d, or chk1-d background) were grown to log phase, shifted to 36°C (for 3 h), irradiated (250 Gy), and allowed to recover for 40 min. At each stage, samples were removed; protein was extracted; and 50 μg (pellet fraction) was analyzed for Mik1-MYC.