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A model indicating two mechanisms linking S phase and mitosis. (Normal S phase, Upper) Intrinsic: Mik1 is synthesized and stable only in S phase. (Lower) Checkpoint control: The Rad3-Cds1 checkpoint maintains HU-treated cells within S phase, as well as directly inhibiting mitosis. HU-arrested cells therefore indirectly require Rad3-Cds1 to accumulate Mik1.
