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. 2000 Feb 25;97(6):2591–2596. doi: 10.1073/pnas.050282397

Figure 4.

Figure 4

5-HT2B receptor activation triggers tyrosine phosphorylation of PDGFR. (A) 5-HT stimulates PDGFR phosphorylation. Cells were incubated with 5-HT (1 μM) for the indicated times. (B) AG 1296 inhibits 5-HT-induced PDGFR phosphorylation. Cells were pretreated with AG 1296 (0.1 μM) for 15 min before stimulation with 5-HT (1 μM) for the indicated times. (C) c-Src depletion inhibits 5-HT-induced PDGFR phosphorylation. After transfection with c-Src sense or antisense oligonucleotides, serum-starved cells were stimulated with vehicle (−) or 5-HT (1 μM; +) for 5 min. (D) PP1 inhibits 5-HT-induced PDGFR phosphorylation. Cells were pretreated with PP1 (1 μM) for 15 min before stimulation with 5-HT (1 μM) for the indicated times. After cell lysis, 250 μg of samples was immunoprecipitated with anti-β-PDGFR antibody. The induction of PDGFR tyrosine phosphorylation was analyzed after immunoblotting with anti-phosphotyrosine antibody (Upper) and, after stripping, the same blot was reprobed with anti-PDGFR antibody (Lower). The position of PDGFR-β is indicated. These data are representative of at least two individual experiments.