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. 2000 Mar 7;97(6):2609–2614. doi: 10.1073/pnas.040576497

Figure 3.

Figure 3

Effect of clones of Dl-expressing cells (green, GFP) on the expression of vestigial boundary enhancer [vg(B)] red in (A, B, E, and F), quadrant enhancer [vg(Q)] red in (C and G), and pattern of VG protein monitored by using an antibody against VG (D and H). In A and E, the vestigial boundary enhancer [vg(B)] is activated in the outgrowth caused by a dorsal clone in both mutant and wild-type cells surrounding the clone (arrow), but not in ventral clones (B and F). In C and G, the Quadrant Enhancer [vg(Q)] is activated in the outgrowth caused by Dl dorsal clones but not in ventral. In dorsal clones the vg(Q) enhancer is repressed in wild-type cells adjacent to the clone but ectopically activated in both mutant cells and wild-type cells [arrow indicates the wild-type cells adjacent to the clone that not express vg(Q) enhancer]. In D and H, the entire wing pouch, including the outgrowth, expresses the VG protein (arrow) (arrowheads point to the d/v boundary). The cells in the outgrowth that do not show VG expression are in a different focal plane.