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PCR specificity. (A) The TRα- and TRβ-specific primers do not amplify the paralogous receptors. Plasmids were used as templates. dCTP (1 μCi) was added to each reaction, and the gel was exposed to film. (B) The TRα- and TRβ-specific oligo probes do not crosshybridize with the paralogous receptor PCR products. TS12 embryo cDNA was used as template. Controls are water blanks.
