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. 2000 Mar 7;97(6):2621–2625. doi: 10.1073/pnas.050566897

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Copyright © 2000, The National Academy of Sciences

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Ectopic expression of Tlx can repress endogenous Pax2. Optic vesicles of stage 9 chick embryos were unilaterally targeted with pCAGGS-Tlx. (A) Embryos were allowed to develop for 12–24 hrs and then fixed and processed for whole-mount in situ hybridization with chick Pax2 antisense RNA probes. The level of Pax2 expression is decreased in the ventral retina of the targeted eye (Left shows lateral view). Embryos electroporated with Tlx-EnR and Tlx-VP were allowed to develop up to day 4 before fixation and processing for histology. (B) Ectopic expansion of pigmented retinal (RP) cells into the optic stalk (OS) region was observed with Tlx-EnR and Tlx (not shown). (C) Embryos targeted with Tlx-VP had a widening of the optic fissure (nonpigmented region).