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. 2000 Mar 14;97(6):2626–2631. doi: 10.1073/pnas.97.6.2626

Figure 4.

Figure 4

Delayed differentiation and improper localization of vascular smooth muscle cells in the ALK1−/− embryos. (A and B) Expression of tg-SM in E9.5 wild-type (A) and ALK1−/− mutant (B) embryos. Note the absence of tg-SM expression in the dorsal aorta (arrow with asterisk) of the mutant embryos (B). (C) Expression of the tg-SM in E10.5 wild-type (Left) and ALK1−/− mutant (Right) embryos. The mutant embryo is severely growth retarded and expresses tg-SM in the anterior part of the dorsal aorta and somites. (D and E) Plastic sections of embryos after X-Gal staining shown in C. Note the dorsal aorta in the wild-type embryo is surrounded by lacZ-positive vascular smooth muscle cells (D), whereas the lacZ-positive cells (*) in the mutant embryo are localized near the dorsal aorta but fail to encircle the vessel (E). cc, common carotid; cv, cardinal vein; da, dorsal aorta; ht, heart; s, somite.