Table 1.
GenBank accession number, fold change, and Entrez definition of the top 30 differentially regulated genes
| Accession no. | Fold | Entrez definition | Group | |
|---|---|---|---|---|
| Genes elevated in LYar cells | ||||
| 1 | M25324 | 66 | Mouse peripheral lymph node-specific homing receptor (MEL-14 antigen) mRNA, complete cds | |
| 2 | AA276043 | 56 | Similar to gb:L10320 fructose-1,6-bisphosphatase | 1 |
| 3 | ET63206 | 45 | Mus musculus mRNA for fructose-1,6-bisphosphatase, partial | 1 |
| 4 | Msa.1236.0 | 44 | Mus musculus thymosin B4 (Ptmb4) gene | |
| 5 | U38252 | 34 | Mus musculus FX-induced thymoma transcript (FXI-T1) mRNA, complete cds | |
| 6 | Msa.14975.0 | 33 | Homologous to sp P17097: zinc finger protein 7 | |
| 7 | Msa.43194.0 | 31 | Mus musculus membrane glycoprotein gene (extracted 3′-UTR) | |
| 8 | Msa.13789.0 | 28 | Homologous to sp P17861: X box binding protein-1 (XBP-1) (TREB5 protein) | |
| 9 | Msa.376.0 | 27 | Mouse adipocyte lipid binding protein gene, complete cds | 2 |
| 10 | U80819 | 24 | Mus musculus glutathione S-transferase homolog mRNA, complete cds | 3 |
| 11 | J05118 | 21 | Mouse mast cell carboxypeptidase A mRNA, complete cds | |
| 12 | Z16078 | 21 | Mus musculus CD53 exon 7 | 4 |
| 13 | Msa.23975.0 | 20 | Homologous to sp P19112: fructose-1,6-bisphosphatase | 1 |
| 14 | Msa.32472.0 | 20 | Homologous to sp P09467: fructose-1,6-bisphosphatase | 1 |
| 15 | U67187 | 20 | Mus musculus G protein signaling regulator RGS2 (rgs2) mRNA, complete cds | |
| Genes elevated in LYas cells | ||||
| 1 | X06086 | 64 | Mouse mRNA for major excreted protein (MEP). Secreted L-cathepsin | 5 |
| 2 | AF023873 | 45 | Mus musculus single-minded 2 (Sim2) gene, exon 11 and complete cds | |
| 3 | d90362 | 43 | Mouse mRNA for cadherin-associated protein (CAP102/α-catenin) | |
| 4 | Msa.547.0 | 39 | Mouse cathepsin L gene, complete cds, clones a-H-ras-1 and RIT-1 | 5 |
| 5 | X53825 | 27 | Mus musculus mRNA for heat stable antigen | 6 |
| 6 | AA407907 | 25 | EST01752 Mouse 7.5 dpc embryo ectoplacental cone cDNA library Mus musculus cDNA clone C0013F05 3 | |
| 7 | Msa.1760.0 | 24 | Mus musculus Src-like adapter protein SLAP mRNA, complete cds | |
| 8 | Msa.30310.0 | 21 | Homologous to sp P06797: cathepsin L precursor (EC 3.4.22.15) (major excreted protein) (MEP) | 5 |
| 9 | Msa.453.0 | 20 | Mouse argininosuccinate synthetase (ASS) mRNA, complete cds | 7 |
| 10 | M31690 | 19 | Mouse argininosuccinate synthetase (ASS) mRNA, complete cds | 7 |
| 11 | C76683 | 19 | Similar to human CYP2D7AP pseudogene for cytochrome | |
| 12 | Msa.13177.0 | 17 | Homologous to sp P24807: signal transducer CD24 precursor (M1/69-J11D heat stable antigen) | 6 |
| 13 | U72680 | 17 | Mus musculus ion channel homolog RIC mRNA, complete cds | |
| 14 | Msa.7690.0 | 16 | Homologous to sp P41732: cell surface glycoprotein A15 | |
| 15 | D10849 | 15 | Mouse mRNA for thromboxane A2 receptor, complete cds | |
Fold changes in gene expression were determined by using Affymetrix software, comparing the mRNA intensity at each gene for LYar and LYas cells. In the upper 15 entries, fructose-1,6-bisphosphatase (group 1), adipocyte lipid binding protein (group 2), the glutathione S-transferase (group 3) previously identified in this system (ref. 2), and CD 53 (group 4) were all elevated 20- to 66-fold in the LYar cells compared with the LYas cells. In the lower 15 entries, LYas cells expressed elevated message levels for the cysteine protease L-cathepsin (group 5), heat stable antigen (group 6), and argininosuccinate synthetase (group 7) compared with LYar cells before irradiation.