Figure 3.

Effect of wild-type and mutant viruses on cell-cycle progression. Human fibroblasts were mock-infected or infected at a multiplicity of 10 pfu per cell with TNwt, TNsubUL69^+pUL69, or TNsubUL69^−pUL69. Cultures were treated with nocodozole and phosphonoacetic acid 12 h later. Cells were harvested, fixed, and stained at either 30 or 48 h after infection, and then they were subjected to FACS analysis. The proportions of cells in G₁, S, and G₂ were determined by using cellquest software. As a control, mock-infected cells that were not treated with drugs (MOCK 0 h) were also analyzed.