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Id-specific CD4+ cells in small and intermediate-sized MOPC315 tumors in TCR-TG mice incorporate BrdUrd. (A) Tumor-bearing mice were given BrdUrd for the last 3 days before analysis. Tumor single cell suspensions were triple-stained with anti-CD4, GB113, and anti-BrdUrd mAbs. Id-specific and nonspecific CD4+ T cell were analyzed for BrdUrd incorporation. (B) Comparison of BrdUrd incorporation in CD4+ T cells in small MOPC315 and J558 tumors (see Fig. 6).
