Figure 3.

Analysis of peripheral B cells. (A) Spleen and bone marrow cells from E₂- and P-treated mice were analyzed for B220 and γ2b expression by flow cytometry. Mean percentages (±SD) are shown. The percentage of γ2b (transgene-positive) B cells in the spleen is greater in E₂-treated mice (n = 5) than in P-treated mice (n = 4) (P < 0.03), but not in bone marrow. (B) The number of anti-dsDNA γ2b B cells was enumerated by ELISPOT. The mean number (±SD) of high-affinity anti-DNA B cells in 10⁵ splenocytes of E₂-treated (n = 6) and P-treated (n = 6) mice is shown. E₂-treated mice contain more high-affinity anti-dsDNA-secreting B cells in the spleen (P < 0.02).