Figure 4.

Vκ-RF light chain nucleotide and amino acid sequences. Nucleotide and amino acid sequences of the germ-line gene are shown in the top line: dashed lines below that denote identical sequences, nonbold letters denote silent mutations, and bolded letters denote replacement mutations; *, missing bases. 11-G5D4 and A10–1E4 utilize the same Vκ-RF and Jκ genes as B15–3C6, but are unmutated and have low apparent affinity for DNA (10⁻⁶ M). B15–3C6 is somatically mutated and has high apparent affinity for DNA (10⁻⁹ M). All three hybridomas were obtained from separate fusions. The B15–3C6 Vκ sequence is available from GenBank (accession no. AF217806).