Figure 4.

Specificity of CTLs generated by autologous fusion cells. (A) T cells were incubated with autologous DC/breast tumor fusion cells in the presence of HuIL-2. The stimulated T cells were cultured with ⁵¹Cr-labeled autologous breast tumor cells (BT) or autologous monocytes (MC) for 5 h at a 30:1 ratio. (B) T cells stimulated with autologous DC/breast tumor fusion cells were incubated with ⁵¹Cr-labeled autologous breast tumor cells, autologous monocytes, MCF-7 cells, ovarian cancer cells (OVCA), or K562 cells at a 30:1 ratio (solid bars). The targets were also preincubated with an anti-MHC class I antibody (W6/32; 1:100 dilution) and then assayed for lysis (hatched bars). CTL activity was determined by ⁵¹Cr release. The results are expressed as mean ± SD of three replicates.