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. 2000 Mar 14;97(6):2719–2724. doi: 10.1073/pnas.97.6.2719

Figure 2.

Figure 2

SDS/PAGE analysis of fractions isolated from sera from six patients, immunized with globo H-KLH, by affinity chromatography on a globo H-agarose column. Lanes 1–6, low pH-eluted fractions from patients 1–6. Lane 7, eluted fraction from patient 3 further purified to remove HSA. Lane S, protein standards (220, 130, 90, 70, 60, 40, 30, and 20 kDa). The migration rates of IgM, IgG, and HSA are indicated. The samples were separated on a 7% polyacrylamide gel under nonreducing conditions and then stained with Coomassie blue.