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Heparin-BSA ELISA. Analysis of epitope expression on wild type (HPV33) and chimeric (HPV33:16BC and HPV16:33BC) VLPs bound to Heparin-coated ELISA plates using type-specific antibodies H16.56E, H33.J3 and H33.B6. Exchange of aa 51–58 (BC-loop of capsid protein L1) results in the loss or gain of reactivity with antibody H33.J3.
