Table 2.

Details of primers and amplicons for each of the 10 evaluated genes.

Gene	Forward Primer Sequence [5'→3']	Position in cDNA	Reverse Primer Sequence [5'→3']	Position in cDNA	Amplicon Length	E%	R2
Act-B	CCATCGGCAATGAGCGGTTCC	3rd Exon	CGTGTTGGCATACAGGTCCTTCC	4th Exon	146bp	97.9	0.997
B2M	TGGCTCCTTTCGTGACCTTGG	1st Exon	GTCGTGAGTAAACCTGAACCTTCG	2nd Exon	96bp	99	0.996
GAPDH	CAAGGCTGTGGGCAAGGTCATC	7th Exon	TTCTCCAGGCGGCAGGTCAG	7th Exon	111bp	97.4	0.995
HPRT1	GTCAAGCAGCATAATCCAAAGATG	6th Exon	GTCTGGTCTATATCCAACACTTCG	7th Exon	84bp	98.1	0.998
PGK1	ACAATGGAGCCAAGTCAG	3rd Exon	CACGCAGTCCTTCAAGAAC	4th Exon	146bp	97.4	0.996
RPL4	CAGACCTTAGCAGAATCTTGAAAAGC	8th Exon	CCTGGCGAAGAATGGTGTTCC	9th Exon	171bp	99.9	0.998
RPS18	CAATTAAGGGTGTGGGGCGAAG	2nd/3rd Exon	TCTTGTATTGGCGTGGATTCTGC	4th Exon	141bp	100.8	0.999
SDHA	TCTTTCCCACCAGGTCACACAC	3rd Exon	CCAGTCGGAGCCCTTCACG	4th Exon	119bp	88.2	0.993
TFRC	ACCATCTCAGTCATCAGGATTG	8th Exon	CATTCTTGTTCTGTGAGGATACC	9th Exon	152bp	98.1	0.998
YWHAZ	AAATGAAAGGAGACTACTACCGCTA	2nd Exon	AGACCCAATCTGATAGGATGTGTTG	3rd Exon	151bp	95.9	0.994

Primers are located in different exons, except for GAPDH, where primers are located in the same exon. E is a measure of real-time reaction efficiency and R² indicates reproducibility of the reaction.