Figure 2. Genetic Organization of ORPHETs and Chimeras Illustrating Conserved Regulatory and Protein-Coding Regions.

Homologous gene regions within each panel share the same coloring. The coding region of each T3SE starts at the beginning of the solid black bar, although the length of the upstream region is not to scale with the coding region. The promoter sequences and ribosome-binding sites are indicated, but not shown to scale. Variable residues in these sequences have been highlighted in gray.

(A) The three HopW1 family members along with HopAE1_PsyB278a in P. syringae share N-terminal homology that extends to the upstream region and includes the ribosome-binding site and hrp box promoter. The hrp box promoter of HopW1–1_PmaES4326 and flanking region, however, is more closely related to that of HopD1_PtoDC3000, although these two proteins are not homologous.

(B) Xanthomonas spp. effectors XopJ and AvrXccE1 along with hypothetical protein XAC3230 share a common N terminus and display conservation upstream of the protein-coding region.