Figure 3.

Control of experimental lung metastases and tumor outgrowth is impaired in Tg mice. (A) Mice were injected i.v. with 3 × 10⁴ B16 cells. The numbers of macroscopic tumor metastases in the lungs were counted on day 14. Anti-NK1.1-treated mice received three i.p. injections (200 μg/injection) of anti-NK1.1 on days −2, +2, and +7. Results are represented as the average number ± SD from four mice per group. (B) Varying numbers of RMA-S cells as indicated were inoculated s.c. into the flanks of WT (seven mice per group, □), Tg (six to seven mice per group, ○) and anti-NK1.1-treated WT (three to five mice per group, ⋄) mice, respectively. Anti-NK1.1-treated mice received five i.p. injections (200 μg/injection) of anti-NK1.1 on days −4, −2, +7, +14, and +21. Palpable tumors were scored twice weekly.