. 2006 Sep 11;103(38):13968–13973. doi: 10.1073/pnas.0606433103

Table 2.

Kinetic properties of WT and mutant CaMs

CaMs	K_D values for Ca²⁺, μM				K_act for CaMKII, μM²	k_p of CaMKII, (sec⁻¹)³
CaMs	K_D1	K_D2	K_D3	K_D4	K_act for CaMKII, μM²	k_p of CaMKII, (sec⁻¹)³
WT CaM	7.9	1.7	35	8.9	0.04	0.96
CaM-C^WT	25	1.6	–	–	5	0.064
CaM-N^WT	28	3.3	–	–	20	0.12

K_D values for Ca²⁺ were calculated as described in Methods from the data in Fig. 3D with the CaLigator program (47) downloaded from www.bpc.lu.se/research/caligator. A model containing four Ca²⁺ sites was used for WT CaM, and one containing two Ca²⁺ sites was used for CaM-C^WT and CaM-N^WT. The concentration of WT CaM required for half-maximal rate of CaMKII autophosphorylation, K_act, is taken from Meyer et al. (16). K_act values for mutant CaMs were determined from the data in Fig. 4B. The intrinsic turnover rate of CaMKII autophosphorylation, k_p, was determined from data in Fig. 5. Confidence intervals (95%) for k_p values were 0.67–1.25 for WT CaM, 0.044–0.084 for CaM-C^WT, and 0.079–0.16 for CaM-N^WT.