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. 2000 Feb 25;97(6):2737–2742. doi: 10.1073/pnas.050583597

Figure 1.

Figure 1

(A) Btk expression in multiple murine bone marrow-derived hematopoietic lineages. Bone marrow cells were immunostained as indicated with FITC-conjugated antibodies for one of the following lineage markers: B (B220+), T, and natural killer (Thy 1.2+) monocyte (Mac1+), granulocyte and mast (Gr1 +), or erythroid (Ter119+). After incubation with anti-FITC-conjugated magnetic beads, marker-positive cells were purified by retention on and elution from a magnetic column. Purified cells were fixed and immunostained with a nonspecific antibody (open histograms) or anti-Btk (filled histograms) followed by PE-anti-rabbit. The number within each graph represents the mean fluorescence intensity of cells in the anti-Btk-immunostained population. (B) Expression of Btk in hematopoietic stem cells and common lymphoid progenitors. Bone marrow cells were immunostained as indicated with antibodies for cell surface proteins defining totipotent hematopoietic stem cells (c-kithi, Sca-1hi, IL-7R−, Lin−), common lymphoid progenitors (c-kitlo, Sca-1lo, IL-7R+, Lin−), or early B lineage cells (B220+, CD43+, IgM−). Cells were purified by FACS, were permeabilized with saponin 0.03% (in Hank's balanced salt solution, 2% FCS, 0.02% sodium azide), and were immunostained with a nonspecific antibody (open histograms) or anti-Btk (filled histograms) followed by FITC-anti-rabbit.