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. 2006 Sep 12;103(38):14217–14222. doi: 10.1073/pnas.0602331103

Fig. 4.

Fig. 4.

PDE4 inhibitor increased urine osmolality in mutant AQP2 heterozygous knockin mice. (A) Urine osmolality of the heterozygous mutant AQP2 knockin mice before and 1 hr after PDE inhibitor administration. Rolipram, a PDE4 inhibitor, significantly increased urine osmolality in heterozygous knockin mice. (B) Immunofluorescence of wild-type and mutant AQP2 before and 1 hr after the administration of rolipram. (C) cAMP content in the papillae before and 1 hr after the administration of rolipram. Rolipram and dehydration increased cAMP content significantly (P < 0.01, n = 4). However, no further increase in cAMP content was observed by rolipram in dehydrated knockin mice. Dehydration was carried out for 24 hr. (D) Western blots of papilla samples from euhydrated knockin mice before and 20 min after the administration of rolipram. The membranes were probed with antibodies against phosphorylated AQP2 (pAQP2), total AQP2, mutant AQP2, and actin.