Fig. 3.

Northern blot hybridization of hsp expression in larval and adult B. antarctica at ambient temperature (4°C) and in response to suboptimal and superoptimal temperatures. The larvae constituitively express their hsps (as documented at 4°C, a temperature typical of summer air temperatures at Palmer Station, Antarctica), but the adults do so only in response to temperature stress. For low temperature exposure, expression was assessed both immediately after the stress was incurred and after a recovery period at 4°C. Expression after heat shock was assessed immediately after the stress. As a control for equal loading, blots were stripped and rehybridized with a 28s RNA probe (GenBank accession no. DQ459549).