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Analysis of cMpl-R-binding Fabs expressed as soluble proteins. FACS analysis of Fabs expressed in bacterial supernatant on 293 EBNA cells +/− cMpl-R transfection. The parental scaffold Fab (anti-TT) used for peptide grafting was tested as a negative control. Representative Fabs containing a single cMpl-R-binding peptide in HC CDR3 (Fabs X0, X1c, and X4b) differ only by flanking amino acids.
