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16K hPRL mutated so as to abolish the hydrophobicity gradient of its tilted peptide shows reduced activity. MBP-16KhPRLmut (■) and MBP-16KhPRL (♦). ABAE cells were treated for 18 h with the indicated protein. Each result is expressed as an enhancement factor (treated vs. untreated cells). Data are means ± SD, n = 3.
