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. 2006 Sep 15;103(39):14390–14395. doi: 10.1073/pnas.0603836103

Fig. 2.

Fig. 2.

Effects of morpholinos on periimplantation conceptus trophoblast growth and differentiation. MAO-std, MAO-5mis, or MAO-env was injected into the uterine lumen on day 8 after mating, and conceptuses were recovered on day 16 (see Materials and Methods for experimental details). (A) Morphology of the conceptuses was examined by using an inverted microscope. Micrographs are shown at the same magnification. Note the retarded growth in the conceptus recovered from a MAO-env-treated ewe. (B) Portions of the conceptuses were fixed in paraformaldehyde, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. (Width of each field of view is 420 μm with the Inset at 85 μm.) (C and D) Trophoblast giant binucleate cells (BNCs) in conceptuses were detected by pregnancy-associated glycoproteins (PAGs) (C) and CSH1 (alias placental lactogen) (D) in the conceptus. Immunoreactive PAG and CSH1 proteins were detected in paraformaldehyde-fixed, paraffin-embedded sections of conceptuses by using a rabbit anti-ovine PAG or anti-ovine CSH1 antibody. (Width of each field of view is 420 μm with the Inset at 210 μm.) Data are representative of conceptuses from all ewes. MTC, mononuclear trophectoderm cell.