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. 2006 Sep 18;103(39):14396–14401. doi: 10.1073/pnas.0602763103

Fig. 2.

Fig. 2.

Expression of the SIPC transcript. (A) Northern blot. Total RNA extracted from the prosomas of B. amphitrite (Ba) and M. rosa (Mr) was hybridized with two probes corresponding to the N-terminal (Left) and the C-terminal (Right) regions of the protein. The RNA ladder is indicated on the left. (B) Expression of B. amphitrite SIPC and EF1 mRNA in different tissues. Lane 1, molecular weight marker (1 kb plus DNA ladder; Invitrogen); lane 2, epidermis; lane 3, egg mass; lane 4, ovary; lane 5, penis; lane 6, gut; lane 7, muscle; lane 8, cirri; lane 9, seminal vesicles. For the no-template control see lane 3 in C. (C) Expression of B. amphitrite SIPC and EF1 mRNA in different development stages. Lane 1, molecular weight marker (1 kb plus DNA ladder; Invitrogen); lane 2, blank; lane 3, no-template control; lane 4, nauplius stage 1; lane 5, nauplius stage 2; lane 6, cyprid (immediately after metamorphosis from the stage 6 nauplius); lane 7, cyprid 2 days after metamorphosis; lane 8, juvenile; lane 9, adult.