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NIK increases total and IgG2a antibody responses against the vector-encoded antigen GFP in vivo. (A) Schematic presentation of AdGFP and AdNIK(GFP) viral vectors. (B–D) ELISA of GFP-specific IgG (B), IgG1 (C), and IgG2a (D) antibody levels in sera of mice 14 days after immunization with saline (■), GFP in CFA (▴), AdGFP (○), or AdNIK(GFP) (▵). Results are shown as mean OD units of five mice per group plotted against serum dilutions.
