Fig. 1.

Chronic arthritis in the loCII-D1CC mouse. To compare D1CC and DBA/1 mice receiving loCII or hiCII, clinical pictures of each mouse were monitored twice weekly and scored (Table 1). Eight-week-old mice were injected with bCII/complete Freund's adjuvant and boosted with bCII/incomplete Freund's adjuvant 3 weeks later (arrows below the graph). (A) loCII-D1CC but not loCII-DBA/1 mice develop polyarthritis, which persists for a longer period. Graphs are as follows: Line with filled circles, D1CC mouse injected with loCII (loCII-D1CC); bold line with filled squares, DBA/1 mice injected with loCII (loCII-DBA/1); dashed line with filled triangles: DBA/1 mice injected with hiCII (hiCII-DBA/1). Eighty-nine percent of loCII-D1CC but only 57% of hiCII-DBA.1 mice developed arthritis. Our clinical criteria focused on heat, swelling, redness, and functional impairment. (B–E) Joints of loCII-D1CC mouse are swollen. Although parental DBA/1 mice had no phenotype on loCII (D and E, fore limbs and hind limbs, respectively), loCII-D1CC mice developed swollen and red extremities on this regiment (B and C, fore limbs and hind limbs, respectively). (F and G) Joints of loCII-D1CC mouse are hot. Thermographs were performed with joints of loCII-D1CC and loCII-DBA/1 mice. Whereas the maximum temperature at the foot reached 25°C in average in the loCII-DBA/1 mouse (G), it reached 28°C on average in the loCII-D1CC mouse at 8 weeks (F). Arrows in F and G point in the direction of thermographs, from arrow point to arrowhead. Numbers below the graph indicate the distance from arrow point in millimeters. The thermal scale with corresponding temperatures (°C) is presented as colors changing from blue to red to the right of G.