Fig. 4.

Joint space narrowing, erosions, and osteoporosis in the loCII-D1CC mouse. (A–D) Progressive joint damage in the loCII-D1CC mouse. Radiographic examination reveals the decline of bone mineral density and joint space narrowing and erosions in the fore limbs at 0 (A), 9 (B), 18 (C), and 39 (D) weeks after the first injection. (E–J) Joint space narrowing, erosions, and osteoporosis in the knee of the loCII-D1CC mouse 24 weeks after the boost. (E and F) CT scans are presented for the distal femur, the knee joint, the proximal tibia, and the fibula. Note the narrowing of the joint space (E, left arrow), erosions, and osteoporosis (E and F, right arrows). None of these changes are observed in the parental loCII-DBA/1 mouse treated likewise (G and H). In sharp contrast, hiCII-DBA/1 mice developed erosions and formed new bone resembling osteophytes 12 weeks after the boost (I and J, arrows). (K) Progressive osteoporosis in the loCII-D1CC mouse. Presented is the comparison of decreased periarticular bone mineral density over time between ldCIl-D1CC and hiCII-DBA/1 mice. Ten sections of cancellous bone measuring 0.1 mm each were analyzed by densitometry of CT scans from both knees in each mouse. Times after the boost are presented below the bar graphs. Data are presented as percentage of bone mineral density of loCII-D1CC (black bars) and hiCII-DBA/1 (gray bars) compared with the untreated parental DBA/1 mouse (WT was given the value of 100%; open bar) over time. Six mice were studied in each group. Error bars reflect SEM. All values were calculated by using data from three or four mice. Student's t test was performed for each value between loCII-D1CC and hiCII-DBA/1 mice (∗, P < 0.05).