Table 5.
Primer sequences for GCSFR sequencing
| Regions | PCR primers | Sequence primers |
|---|---|---|
| I | F1-1 (8512): CTT GAA GGG TGA GAC AGG AAG GTG | FS1-A (8552): GGA GTA CAA AGG CCT AGA TTA TAG C |
| R1-1 (10991): CTT CTG TGC CTC TGT CTC TAG GTC | FS1-B (10295): CAG ACA AGG TGA CAA CAG AGG AAC | |
| 8529–10970 | F1-2 (8529): GAA GGT GAG GCC TCT GAG TCT AGG | RS1-A (9024): GAA TAC AGG CGT GAG CCA ACG TGC |
| R1-2 (10970): G TCT TCC AGT GTC TTC TTG TCT GTC | RS1-B (10948): GT CGC TAG TTC TGT GTT TCC CTC TCC | |
| II | F2-1 (11477): CAT AGA GAT CGA ACC ACA GAA ACG | FS2-A (11527): GAA CCA CAG ACA GGG AGA CAA GAG |
| R2-1 (10034): GAA CTC CTG ACC TCG GAT GAT CTG | FS2-B (12560): CTC CGT TTG TAT GAT CCA TCC ACC | |
| 11499–13000 | F2-2 (11499): CGT GTC ATT GGC AAC GTG GAC AGA TG | RS2-A (12332): CTC CCA GAC CTG TTG GAG TCC TAA G |
| R2-2 (13000): CCT CCC AAA GTG CTG GGA TTA CAG | RS2-B (12950): GGA CTA GAT TT AAC CCA GGC AGT C | |
| III | F3-1 (14307): CTA CTT TAC AGA TGA GGA AAC TGA G | FS3-A (14381): CAG GAT TTG AAC CCA GGC TTC TGG |
| R3-1 (15308): CTG TAG GGA TCC AGT GTA AGC CAG | ||
| RS3-A (15234): GTT CCC TAT ACT TCT GAT TGC TGG | ||
| 14332–15263 | F3-2 (14332): GCT AAG ATG TTG ACC TAC CAA AGG | |
| R3-2 (15263): CAG GGC TGG AAG TAT GGT AGG AAG | ||
| F4-1 (15917): GAT GGA ATC TGG ATT AAA TCC CTG G | FS4-A (15997): GTG TCT GGG AAG CCA CAA GAA GTC | |
| IV | R4-1 (18330): GAA GAA AGG ACC AAC TCT GAG AAG | FS4-B (16561): GGA CTG ACT TTG AAT CCC CTG GTC |
| FS4-C (17362): CAC CAG TAA CAA CAG TTG AAT GTC | ||
| 15962–18314 | F4-2 (15962): GAG TCC CCT TGG ACT CTT TTC TCA C | |
| R4-2 (18314): CTG AG AAG TTT CCC CCT GAC TGC TG | RS4-A (16553): CTC TCA AAA TCA GCA TCC TTT GGG | |
| RS4-B (17146): GCT TCA GAA GGT GTC CCT TCA CTG | ||
| RS4-C (18276): AAA TAA CAG AGA CTC AGG TAC ACC |