Fig. 3.

Western blot for protein expression of K-Ras, Cox-2, Akt1/2, and pAkt (S-473) in orthotopic xenografts of H460 human NSCLC after treatment with GHRH antagonists MZ-J-7-138 (10 or 20 μg/day), JV-1-92 (10 μg/day), or untreated. Four representative tumors from each group are shown, and the experiments were repeated at least three times. Protein levels were normalized to β-actin protein (42 kDa) and are expressed as percentage of control values as shown in Table 2.