Figure 3.

In-frame κ light chain repertoire is more restricted in mature naive B cells than in immature B cells from three daisy (V_HJ558 Transgenic) mice. In-frame κ light chain joins to J_κ2 from immature (population E, white bars) and mature (population F, black bars) B cell populations were amplified, cloned, and sequenced. This figure is as described for Fig. 2 a–c. (a–c). In all three Daisy line mice analyzed, V_κ1 family member number 82 (marked with an asterisk) was more prevalent in the mature B cell population than the immature B cell population, and this enhancement was significant (P < 0.003) (15). (a) E, n = 17; F, n = 31. (b) E, n = 33; F, n = 41. (c) E, n = 48; F, n = 46. (d) Tabulation of all V_κ-J_κ2 sequences from the Daisy line for immature (E) and mature (F) cells is shown. The horizontal axis is labeled with as above whereas the vertical axis depicts total number of sequences with the immature numbers normalized to be equivalent to the mature numbers (n = 118 for both populations). V_κ1 sequence 82 (marked with an asterisk) represents 6 out 118 sequences (5.1%) in immature B cells and represents 21 of 118 (17.8%) sequences in mature B cells. This enhancement is highly significant (P < 0.003). (e) The same sequencing protocol as in Fig. 2 was applied to a nontransgenic control mouse of the same background as the Meg line. Population E, white bars; population F, black bars. E, n = 46; F, n = 40.