Fig. 4.

Time course of glycoprotein complex formation in transfected cells. 293 cells were transfected with expression plasmids for gD, gB, and gH·gL and twice the amount of HVEM plasmid. At the indicated hour p.t., cells were harvested and HVEM or gD were immunoprecipitated (IP). Immuno- and coimmunoprecipitated proteins were analyzed by WB to gD and gB, and, for the 24-h lane, to gH and gL. Complex formation was detectable at 18 and 24 h p.t. but not at 36 h p.t.