Glycoprotein complex assembly requires a gD receptor. The receptor-negative CHO cells were transfected with expression plasmids for gD, gB, and gH·gL with HVEM (+HVEM), with nectin 1 (+Nec), or with no receptor (+No R). gD was immunoprecipitated with pAb R8. Immuno- and coimmunoprecipitated proteins were analyzed by WB, as indicated. It can be seen that in the absence of receptor, no gB was coimmunoprecipitated.