Fig. 3.

5α-R type I is expressed in pyramidal neurons of the hippocampus. (A) 5α-R type I mRNA colocalizes with VGLUT2 in CA1 pyramidal layer. (A1) 5α-R type I mRNA, green. (A2) VGLUT2 protein, red. (A3) Merge of A1 and A2. (B) VGLUT2 antiserum specificity. Western blot of mouse brain extract incubated with VGLUT2 antibody (B1) and VGLUT2 antibody preabsorbed with VGLUT2 immunizing antigen (B2) (see Materials and Methods). Molecular markers are indicated at the right. (C) 5α-R type I mRNA does not colocalize with S100β in the CA1 area of the hippocampus. (C1) 5α-R type I mRNA, green. (C2) S-100β protein, red. (C3) Merge of C1 and C2. O, stratum oriens; R, stratum radiatum. (Scale bars, 20 μm.)