Fig. 2.

Interaction between R. solanacearum GALAs and Arabidopsis ASKs. (A) Y2H among BD-GALA6, BD-GALA6ΔF-box, and AD-ASK1. Serial 10× dilutions were spotted from left to right on nonselective medium (−LT) and selective medium lacking His (−LTH). (B) Western blot probed with anti-ASK1 antibody after GST pull-down of Arabidopsis total protein extract. Equal amounts of GST, GST-GALA6, and GST-GALA6ΔF-box (as estimated by PAGE/Coomassie, data not shown) were incubated with total protein extract from Arabidopsis seedlings expressing 6myc-tagged ASK2 (23). (C) Y2H interaction pattern between ASKs and GALAs. Positive interactions were observed only when the BD was fused to GALA and the AD was fused to ASK.