Figure 2.

(A) Dose–response (Left) and time course (Right) of the effect of PDTC on CCR2, CCR5, and CXCR4 mRNA expression. For the dose–response analysis, total RNA was purified from monocytes incubated for 4 h with PDTC at the indicated doses. In kinetic analysis, the total RNA was purified from fresh human monocytes incubated with 50 μM PDTC for the indicated times. Results in A are representative of three separate experiments. (B) Effect of PDTC on the CCR2, CCR5, and CXCR4 mRNA half-lives. ActD (1 μg/ml) was added to fresh human monocytes in the presence or absence of PDTC (50 μM), and total RNA was extracted at different times as indicated. Results in B are representative of two different experiments.