Figure 4.

(A) Inhibition of monocyte migration by PDTC. Fresh human monocytes were incubated with 50 μM PDTC and assayed for chemotactic response after different periods as indicated. Results in A are representative of five different experiments. (B) Effect of PDTC on CCR2, CCR5, and CXCR4 surface expression. Surface expression was determined by flow cytometry, using human anti-CCR2, anti-CCR5, or anti-CXCR4 antibodies. Dotted line, irrelevant mAbs; continuous line, cells stained with anti-CCR2, anti-CCR5, or anti-CXCR4 as indicated. Results in B are representative of four different experiments. FL1-H, fluorescence one height; FL2-H, fluorescence two height.