Figure 5.

(A) Effect of ROI on CCR5 surface expression. Fresh human monocytes were incubated with the ROI-generating system X/XO (X, 50 μM; XO, 1 milliunit/ml) for 18 h. Surface expression was determined by flow cytometry, using human anti-CCR5 antibody. Dotted line, irrelevant mAbs; continuous line, cells stained with anti-CCR5 as indicated. Results in A are representative of four different experiments. (B) Fresh human monocytes were incubated with X/XO or H₂O₂ and assayed as indicated for chemotactic response after 18 h. Results in B are representative of five different experiments. (C) Effect of ROI on MCP-1 and MIP-1β secretion by monocytes. Cells were activated with X/XO for 18 h, and supernatants were assayed by ELISA for MCP-1 and MIP-1β proteins. Results in C are representative of three separate experiments.