Table 1.
Oligonucleotide primers for PCR amplifications and sequence analyses
| Primer | Oligonucleotide sequence (5′ → 3′) |
|---|---|
| 1A (exon 36) | CTTCAGTGGACCTTCATAATGC |
| 1B (exon 43) | CCATACAAACTATCTGGCTCC |
| 2A (exon 29) | CAGAGATTGTGGTGGAGTTATTG |
| 2B (exon 36) | GCATTATGAAGGTCCACTGAAG |
| 3A (exon 42/43) | CTGGAATAAGTTTACAGGATCTTC |
| 3B (exon 51) | GATGATTTCATGTAGTTTTCAATTC |
| 3A1 (exon 45/46) | CATTGCACTTCCGTCAGC |
| 3A2 (exon 48) | CTATCATGGCTCTACGCAC |
| 3B3 (exon 49) | AATACTTGTGCTTCTTCCAG |
| 4A (exon 56) | AAGATGTTGTTGTCCCTACTATG |
| 4B (exon 65) | AAGGCTGAATGAAAGGGTAATTC |
| 4A2 (exon 64) | CCTTCTATATGATCCACTC |
| 4B3 (exon 61) | TCTGTACATGTCTATCACC |
| 5A (exon 51) | GATGGAGAAAGTAGTGATGAGC |
| 5B (exon 57) | AGTCACCAGATTTCCATATTCTC |
| 5A2 (exon 54/55) | GCTCTCAGCTTGATGAGG |
| 5B1 (exon 52) | TACCCACATATCATGTTC |
| 6A (exon 22) | CTAGGTCAAAGCAATATGGACTC |
| 6B (exon 30) | CATGCGATGGAAAATGAGGTG |
| 6A2 (exon 28) | ACATCTGGTGATTAGAAGTC |
| 7A (exon 36) | GAGGTCAAACCTAGAAAGCTCAC |
| 7B (exon 22/23) | CCTCTCCTTTGTTAGATGCC |
| 7A1 (exon 13) | TGCCTCCAATTCTTCACAG |
| 7B5 (exon 19) | ATGGTACTTTGGCTCTCTCC |
| 8n1A (exon 3) | AGGCATACATCACAATTTGG |
| 8n1B (exon 12) | TTGCTCAGAACTTATACCACG |
| 8A5 (exon 6) | CAGCCTCAACACAAGCCTC |
| 8B4 (exon 8) | GCATCCTTTGGTAACAGCAT |
| 29A (intron 28) | AGATTGTGGTGGAGTTATTGA |
| 29B (intron 29) | TTAAAAAGAGTGATGTCTATAA |
| 48A (intron 47) | CTCTTGCTTACATGAACTCTA |
| 48B (intron 48) | AAGAGGTAAGATGACATAGTT |
| 59A (intron 58) | AGGTCAACGGATCATCAAAT |
| 59B (intron 59) | TTAATTTTGGGTGTCACTC |
Primer sets 1A/B, 2A/B, 3A/B, 4A/B, 5A/B, 6A/B, and 7A/B and primer pairs 29A/B, 48A/B, and 59A/B have been described previously (42, 43). 8n1A, 8n1B, 3A1, 3A2, 3B3, 4A2, 4B3, 5A2, 5B1, 6A2, 7A1, 7B5, 8A5, and 8B4 were derived from cDNA sequence data from the ATM gene (GenBank accession no. U33841) (44).