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. 2006 Apr;8(4):290–301. doi: 10.1593/neo.05694

Figure 6.

Figure 6

Adhesion of prostate tumor cells to extracellular matrix proteins depends on CXCR4. DU-145 (A) or LNCaP (B) cells treated with scrambled siRNA, or DU-145/LNCaP cells whose CXCR4 was knocked down were activated with 500 ng/ml CXCL12 and then added to immobilized fibronectin, laminin, collagen, or nonspecific poly-l-lysine (PLL) at a density of 0.5 x 106 cells/well for 60 minutes. Nontreated cells served as controls. Nonadherent tumor cells were washed off in each sample; the remaining cells were fixed and counted in five different fields (5 x 0.25 mm2) using a phase-contrast microscope. Mean values were calculated from five counts. Specific adhesion capacity (background adhesion on a plastic surface was subtracted from adhesion to matrix proteins) is depicted as counted cells per square millimeters. One of six representative experiments is shown. **Significantly different from controls (P < .01).