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Western blot of protein extracts from C6, C6-stb-tk+, and C6-stb-sr39tk+ cells. Staining with TK antiserum yields qualitatively similar signals for C6 cells expressing wild-type HSV1-tk (middle blot) and C6 cells expressing HSV1-sr39tk (right blot). Control C6 cells (left blot) show no significant signal. β-Actin expression is similar for all three cell lines. Total protein concentration used was 10 μg per lane for each cell type.
