Figure 4.

FPCV accumulation in cells infected with Ad-CMV-HSV1-sr39tk. C6 cells were infected with five different viral titers of Ad-CMV-HSV1-sr39tk. Cells were subsequently exposed to FPCV for 120 min, then harvested and assayed for FPCV accumulation, HSV1-sr39tk mRNA, and GAPDH mRNA levels. (Top) HSV1-sr39tk mRNA levels (normalized to GAPDH levels) as a function of viral titer. (Middle) FPCV-normalized accumulation as a function of viral titer. The units of normalized FPCV accumulation are: [dpm cells/dpm medium at time zero/μg of total protein]. (Bottom) FPCV-normalized accumulation as a function of HSV1-sr39tk mRNA levels (normalized to GAPDH).