RT-PCR analysis of various wnt and fz
isoforms in RA and OA tissue specimens. Total RNA was extracted from
five RA and five OA synovial tissue samples. RT-PCR was performed by
using 1 μg of RNA from each sample with different primers for the
specified wnt and fz isoforms as well as
β-actin. (A) fz2, (B)
fz5, (C) wnt1,
(D) wnt5a, (E)
wnt10b, (F) wnt13, and
(G) β-actin. The PhiX 174 DNA standard is shown in the
first lane of each gel.