RT-PCR analysis to identify expression of wnt5a,
IL-6, IL-8, and IL-15 in RA fibroblasts compared with normal synovial
fibroblasts. Lane 1, DNA standard PhiX174; lane 2,
wnt5a-specific RT-PCR product from rheumatoid synovial
fibroblast (RA 508); lanes 3–5 and 6, IL-6-, IL-8-, IL-15-, and
G3PDH-specific RT-PCR products from RA 508; lanes 7–11,
wnt5a-, IL-6-, IL-8-, IL-15-, and G3PDH-specific RT-PCR
products from RA 498; lanes 12–16, wnt5a-, IL-6-,
IL-8-, IL-15-, and G3PDH-specific RT-PCR products in normal synovial
fibroblasts.